Abstract
It has been shown by immunohistochemical studies that αB-crystallin accumulates in the reactive and neoplastic glial cells in a variety of pathologic situations. However, the molecular mechanism for the induction of αB-crystallin in diseased brains is still unknown. Since any destructive brain lesions cause an abnormal elevation in the potassium (K +) concentration of the extracellular space, which disturbs the regulatory mechanism of glial cell volume, we investigated the influence of elevated extracellular K + on the expression of αB-crystallin in glial cells. The treatment of rat C6 glioma cells with augmented K + in the culture media induced an accumulation of αB-crystallin mRNA in a dose-dependent manner and an accumulation of the αB-crystallin as well. Furthermore, an overexpression of αB-crystallin in the C6 transformant transfected with a rat αB-crystallin cDNA conferred a resistant phenotype against the insult of elevated extracellular K + on the glioma cells. Thus, αB-crystallin may contribute to the survival of reactive glia in the presence of a high extracellular K + concentration.
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