Abstract
The bcl-2 protooncogene has been shown to protect haemopoietic precursors from programmed cell death after the removal of interleukin-3 (IL3). In the present report we show evidence that overexpression of bcl-2 in the pre-B-cell line BAF3 protects cells from apoptosis induced by treatment with the thymydilate synthase inhibitor 5′-fluor,2′-deoxyuridine (FDUR) in the presence of IL-3. Dose-response experiments analyzing the dependence of cell death on drug concentration indicated a marked resistence of BAF3 bcl-2 to FDUR treatment. Cleavage of DNA into oligonucleosome-length fragments, a characteristic of apoptosis, was observed in BAF3 cells and inhibited in the cells overexpressing bcl-2. We have determined variations in the dATP and dTTP pools after FDUR treatment. Interestingly, no differences were found between both cells in the kinetics of changes in dNTP pools. Therefore, the protective effect of the Bcl-2 protein on apoptosis induced by dNTP unbalance must be ascribed to a step downstream of perturbations in the synthesis of DNA precursors and before activation of endonucleolytic cleavage of chromatin.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
