Abstract
Methods: We measured blood pressure (BP) in 40 spontaneously hypertensive rats (SHRs) at 14:00–16:00 every day. Using gene sequencing to check SHR beta1-adrenergic receptor (beta1-AR) genotype; real-time quantitative reverse-transcriptase polymerase chain reaction (RQ-PCR), real-time reverse transcriptase-polymerase chain reaction (RT-PCR) to analyze myocardial mRNA expression of beta1-AR gene; methylation specific PCR (MSP) and bisulfite sequencing to detect methylated status in beta1-AR gene; protein expression of beta1-AR were identified by western blot.
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