Barrientosiimonas endolithica sp. nov., isolated from pebbles, reclassification of the only species of the genus Tamlicoccus, Tamlicoccus marinus Lee 2013, as Barrientosiimonas marina comb. nov. and emended description of the genus Barrientosiimonas.

Abstract

Strain JC268(T) was isolated from pebbles collected from a dam located in Lalitpur, Uttar Pradesh, India. Cells of strain JC268(T) were coccoid, appeared in pairs/triads/tetrads or short chains and were Gram-stain-positive, non-spore-forming, non-motile and obligately aerobic. Strain JC268(T) was catalase- and oxidase-positive and utilized citrate for growth. The genomic DNA G+C content of strain JC268(T) was 65.3 mol%. The cell-wall peptidoglycan contained L-lysine-L-serine-D-aspartic acid as interpeptide bridge with the type A4α. The major menaquinone was MK-8(H4). Major (>10%) fatty acids were iso-C16 : 0, iso-C16 : 1H and anteiso-C17 : 1ω9c. Diphosphatidylglycerol, phosphoglycolipid, phosphatidylinositol, glycolipid, four unidentified lipids, an amino lipid and phospholipid were the polar lipids of strain JC268(T). EzTaxon-e blast search of 16S rRNA gene sequences showed that strain JC268(T) has highest similarity to Barrientosiimonas humi 39(T) (98.65%) and Tamlicoccus marinus MSW-24(T) (97.8%) of the family Dermacoccaceae. Genome reassociation (based on DNA-DNA hybridization) of strain JC268(T) with Barrientosiimonas humi CGMCC 4.6864(T) ( = 39(T)) and T. marinus KCTC 19485(T) ( = MSW-24(T)) yielded values of 32.5 ± 2% and 27.3 ± 2%, respectively. Based on the data from phylogenetic and polyphasic taxonomic analyses, strain JC268(T) represents a novel species of the genus Barrientosiimonas for which the name Barrientosiimonas endolithica sp. nov., is proposed. The type strain of Barrientosiimonas endolithica is JC268(T) ( = KCTC 29672(T) = NBRC 110608(T)). Our data suggest that T. marinus should be reclassified within the genus Barrientosiimonas. Thus, a reclassification is proposed for T. marinus, the type and only species of the genus Tamlicoccus, as Barrientosiimonas marina comb. nov., which implies the emendation of the description of the genus Barrientosiimonas.