Abstract
Barley is an alternative host for the rice blast fungus Magnaporthe oryzae but is resistant to Magnaporthe species associated with the grass genera Pennisetum and Digitaria. The latter cases are examples for nonhost resistance which confers effective and durable protection to plants against a broad spectrum of pathogens. Comparative transcript profiling of host and nonhost interaction revealed an early and pronounced change in gene expression in epidermal tissue of barley infected with a Magnaporthe nonhost isolate. Interestingly, this set of genes did not overlap considerably with the transcriptional response of barley against nonhost rust or powdery mildew isolates. For a functional testing of candidate genes a combined approach of virus-induced gene silencing (VIGS) and subsequent pathogen challenge was established. As anticipated, VIGS-mediated down-regulation of Mlo-transcripts led to higher resistance against Blumeria graminis f.sp. hordei and enhanced susceptibility against M. oryzae.
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