Abstract
Abstract The baseplate of wild-type T4 phage carries a small number (probably 6) of short tail fibers in addition to the well known long tail fibers and tail pins. Short tail fibers are missing from defective T4 particles (12 − particles) lacking only the product of T4 gene 12 and are restored by in vitro complementation. Phage are neutralized by an antiserum swept with 12 − particles; that neutralizing activity is in turn quantitatively blocked exclusively by soluble 12P or by particles that carry 12P. 12 − particle-swept antibody aggregates phage through their short tail fibers. It can be concluded that these fibers are the product of gene 12 . Antibody blocking experiments and in vitro complementation confirm the pleiotropic role of gene 57 product in phage T4 morphogenesis: 57 − particles are deficient in 12P as well as in long tail fibers.
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