Abstract
Autogenous splenic implant seems to be the only alternative for preservation of splenic tissue after total splenectomy. This work was carried out to analyze the morphologic regeneration of autotransplanted splenic tissue in Wistar rats and to determine the bacterial phagocytic function of their macrophages. We utilized an experimental model with thirty-two rats, of both sexes, submitted to total splenectomy combined with autotransplantation in greater omentum of slices of the whole spleen mass. The animals were divided into two groups: I--young rats weighing 100 to 150 g; and II--adult rats weighing 250 to 300 g. Sixteen weeks later animals were intravenously inoculated with a suspension of Escherichia coli AB1157. Twenty minutes after inoculation, the animals were sacrificed and the splenic autotransplants were removed for morphological study. There was regeneration of autotransplanted splenic tissue in all animals. A similar morphological aspect among all animals was observed, with splenic tissue showing red and white pulps, lymphoid follicles, and marginal zone, with a moderate architectural disarrangement. Macrophages containing gram-negative bacterial aggregates as well as macrophages with hemosiderin pigments within the cytoplasm were observed. Blood vessels showed preserved walls, with no signs of vasculitis or thrombosis. The present results suggest that autogenous splenic implants in the greater omentum of the rat acquire the macro- and microscopic architecture of a normal spleen, with reduced dimensions, and preserve bacterial phagocyte function.
Highlights
The technical aspects concerning heterotopic splenic autotransplant have been widely reported on the literature
Experimental studies investigating the immunoprotector effect of autogenous splenic implants are based on animal exposure to several bacterial species, with evaluation of the following factors: bacterial clearance from the bloodstream (Patel et al, 1986; Cooney et al, 1979; Brown et al, 1981; Malangoni et al, 1988), mortality due to the sepsis caused by the microorganisms (Cooney et al, 1979; Schwartz et al, 1978; Livingston et al, 1982; Malangoni et al, 1985), as well as the potential benefits of immunization combined with splenic autotransplantation (Leemans et al, 1999; Cooney et al, 1979)
This study aims to verify if the macrophages of autogenous splenic implants phagocyte Escherichia coli in rats
Summary
The technical aspects concerning heterotopic splenic autotransplant have been widely reported on the literature. This is a simple procedure not associated with significant complications (Tavassoli et al, 1973; Pabst & Reilman, 1980; Patel et al, 1981, 1986; Millikan et al, 1982; Moore et al, 1984; Nielsen et al, 1984; Pabst & Karam, 1986; Thalhamer et al, 1986; Büyükünal et al, 1987; Iinuma et al, 1992; Timens & Leemans, 1992; Petroianu et al, 1993, 2000; Leemans et al, 1999; Resende & Petroianu, 2001). Other elements that apparently return to normal are antibody production against pneumococci polysaccharides, and the immunoglobulin levels (notably IgM), complement, platelets, and lymphocytes (Patel et al, 1981; Moore et al, 1984; Nielsen et al, 1984; Büyükünal et al, 1987; Iinuma et al, 1992; Petroianu et al, 2000; Leemans et al, 1999; Resende & Petroianu, 2001)
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Schedule a callMore From: Brazilian journal of biology = Revista brasleira de biologia
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