Bacterial dynamics and functions driven by bulking agents to mitigate gaseous emissions in kitchen waste composting

Abstract

This study investigated the impacts of different bulking agents (i.e. garden waste, cornstalks, and spent mushroom substrates) on bacterial structure and functions for gaseous emissions during kitchen waste composting. High-throughput sequencing was integrated with functional Annotation of Prokaryotic Taxa (FAPROTAX) to decipher the bacterial structure and functions. Results show that adding cornstalks constructed a more complex and mutualistic bacterial network to enhance organic biodegradation. This scenario, however, aggravated the emission of ammonia and hydrogen sulphide with the enrichment of the genus Bacillus and Desulfitibacter at the thermophilic stage of composting to facilitate ammonification and sulphur-related respiration, respectively. By contrast, spent mushroom substrates facilitated the proliferation of the genus Pseudomonas to promote nitrate reduction at the cooling stage, leading to considerable emission of nitrous oxide. Compared to these two agents, garden waste contained less easily biodegradable substances to limit bacterial mutualism, thereby reducing gaseous emissions in composting.