Abstract
BackgroundAdherence of bacteria to teeth surface is considered an important step in the development of caries and the use of fissure sealants is crucial for the prevention of caries in occlusal surfaces of molars and premolars. The aim of this study was to investigate and compare the adherence of Streptococcus mutans to different fissure sealants, after acidic drink exposure.Material and MethodsThe tested materials were Fissurit, Fissurit FX, Grandio Seal, Fuji Triage, Constic. Bacterial suspension was deposited onto each material and the adhesion was evaluated trough the colony forming units (CFUs) determination with or without acidic drink exposure.ResultsThe tested materials showed different behaviors with significant differences. Bacterial adherence values of the untreated materials were very dissimilar: Fuji Triage and Constic materials showed the better results (P<0.05).ConclusionsSurface alteration after acidic drink exposure, changed the bacterial adhesion (except for Grandio Seal): Fissurit, Fissurit FX and Fuji Triage decreased their susceptibility to be colonized by S. mutans (P<0.05); on the contrary, Constic increased up to ~4 times the bacterial adhesiveness respected to the untreated control (P<0.05). Key words:Acidic drinks, bacterial adhesion, fissure sealants, Streptococcus mutans.
Highlights
Fissure sealants are widely used in dentistry for the prevention of caries in occlusal surfaces of molars and premolars and their effectiveness has been proved in many studies [1,2,3,4]
The null hypothesis of the study was that there are no significant differences on bacterial adhesion values among the different fissure sealants after exposure to acidic drink
The ability of S. mutans strain to adhere to different sealant materials with or without soft drink treatment, are displayed in in Table 2 and in Fig. 1 as Colony Forming Units (CFU)
Summary
Fissure sealants are widely used in dentistry for the prevention of caries in occlusal surfaces of molars and premolars and their effectiveness has been proved in many studies [1,2,3,4]. The formation of dental plaque is a complex phenomenon involving microbial adhesion to oral surfaces; the plaque composition is an ensemble of salivary conditioning film and the initially adhering bacteria. The aim of this study was to evaluate and compare adhesion of Streptococcus mutans on different fissure sealants after acidic drink exposure. 1 mL of the bacterial suspension (107 bacteria/mL) was seeded onto each sample placed at the bottom of a 24-well plate (Celbio, Milan, Italy) and incubated in static conditions at 37°C for 4 hours. Two-tailed P values of 0.05 were considered statistically significant
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