Abstract

The embryonic axis plays a critical role in inducing expansion and chlorophyll formation in cotyledons of germinating yellow lupin seed, and cytokinins would appear to constitute an important part of the stimulus emanating from the axis. Externally applied cytokinins (6-benzylaminopurine >;>; zeatin >; dihydrozeatin) completely replaced the embryonic axis while gibberellic acid was ineffective. The two optical isomers of dihydrozeatin differed in activity, the unnatural form (R) being more active than the natural form (S). The cotyledons appeared to respond mrkedly to supplied cytokinin only after reaching a certain stage of development (3–5 days after the start of germination), reflecting development of sensitivity to cytokinin or of an ability to utilize the primary response evoked by cytokins.

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