Abstract
The aim of study was to detect H5N1 virus in wild geese in Qinghai Province in 2012. The work was provided according to WHO and OIE guidelines. In 2012, we collected 532 samples from wild geese of two species: Bar-headed Goose (Anser indicus) and Graylag Goose (Anser anser). We analyzed samples by chicken embryo inoculation and PCR. No avian influenza viruses were isolated. History of HPAI H5N1 shows obvious importance of Central Asian region in its spreading. The outbreaks of the H5N1 Highly Pathogenic Avian Influenza (HPAI H5N1) were reported in wild birds at the Qinghai Lake since 2005. This area seems to be key point for H5N1 avian influenza surveillance in wild birds. We did not find viruses although H5N1 cases in poultry were reported from 5 provinces of China in 2012. Annual surveillance is required for early AIV detection in this region.
Highlights
Pathogenic H5N1 influenza viruses (HPAI H5N1) in spite of intensive control measures are known to be serious threat to humans
Swabs and feces samples were tested for influenza viruses by inoculation into the allantoic cavity of 10-day-old embryonating specific-pathogen-free chicken eggs according to standard procedures and by real-time Reverse transcription (RT)-PCR [11]
Swabs and feces samples were tested for influenza viruses by real-time RT-PCR first
Summary
Pathogenic H5N1 influenza viruses (HPAI H5N1) in spite of intensive control measures are known to be serious threat to humans. Since 2003, the number of human cases of avian influenza A (H5N1) reported from 15 countries worldwide is 644, of which 381 were fatal (as of 8 November 2013) [1]. (2014) Avian Influenza H5N1 Surveillance in Geese of Qinghai Province, China (2012). History of HPAI H5N1 shows obvious importance of Central Asian region in its spreading [2]-[8]. Virus has been reported in Qinghai-Tibetan Plateau since 2005 and caused numerous outbreaks in different wild birds and poultry [2] [4]-[6]
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