Avian haemosporidian diversity and transmission across birds and mosquitoes in Botswana.

Mangrove forests throughout the world are recognized as stopover sites by many migratory birds – including waders – as they contain ample food resources such as invertebrates, fruits, and flowers that are beneficial to most birds. In connection, the Talabong Mangrove Forest located in Bais, Negros Oriental, Philippines is recognized as a wildlife sanctuary, but no recent data on birds could supplement this. Thus, this study aims to assess the distribution patterns of migratory and resident birds in the said site during the southward migration which encompasses both wet and dry seasons. Also, the investigation determines whether Talabong mangrove could function as a stopover site for migratory birds. To examine the community structure, the populations of resident and migratory bird species were assessed for species composition, diversity, density, and abundance in three sampling periods: pre-migration, migration, and post-migration. The line transects and point-count methods were employed with four transect lines laid out on the study site. Each line stretched a kilometer long with a 250-m interval with five points for point-counting and was visited on each sampling period. Points were marked for a consistent sampling. For each sampling period, results showed that the birds’ abundance, diversity, and density remained consistently high throughout each sampling. Specifically, the species composition and the number of resident birds did not seem to alter and remained high as well. On the other hand, migrants had an abrupt increase during migration sampling and decreased towards the end. But similarity analyses suggest species composition remained approximately similar for the migrants. The results showed that Talabong mangrove forest contained some migratory species – making it a decent stopover site, although not as many migrants as in other protected mangroves and wetlands in the Philippines. The resident birds occupying the site may also have served as cues for the migrants.

We quantified resident and migrant bird attendance at army ant swarm raids (n = 48) in a neotropical montane forest. All observations were during seasons when Nearctic migrant birds are present. Bird species differed in army ant raid-attending behavior. Resident bird species attended 2 to 54% of raids, while migrants attended at lower maximum frequencies (2 to 21% of raids attended per species). Some resident and migrant bird species attended raids more frequently than expected based on capture rates in mist-net studies and point-count density surveys. Army ant raid attendance may be a regular element of foraging behavior for some resident species, and important in the wintering ecology of some Nearctic migrant species. The bird species that attended raids most frequently were predicted to show behavioral specializations for exploiting army ant swarms. Eight resident bird species (but no migrants) performed a specialized behavior, bivouac checking, by which birds sample army ant activity. Resid...

Background: Turkey is home to various resident and migratory wild bird species. The aim of the present study was to investigate the ectoparasites found on 188 injured or rescued resident and migratory wild birds from Bursa and surroundings between 2015 and 2019. Methods: Sampled birds were examined for different ectoparasites and all of the collected parasites were placed into tubes containing 70% ethyl alcohol. After mounting onto slides or fixing onto a plate, each parasite was identified to species using a light or stereo microscope. Result: Results revealed that 88 (46.8%) of the examined wild birds were infested with one or two of 3 different species of ectoparasites. The species identified were 38 lice, three ticks and two flies. The lice were highly prevalent (40.4%) species than the flies (2.1%) and ticks (2.1%). The results also first geographically documented the lice and ked fly species as follows, with additional new host records: Fulicoffula gallinula and Pseudomenapon pilosum in the common moorhens (Gallinula chloropus); Ciconiphilus decimfasciatus in the grey heron (Ardea cinerea); Saemundssonia clayae in the Eurasian woodcock (Scolopax rusticola); Ardeicola ixobrychae in the common little bittern (Ixobrychus minutus) and the ked fly as Ornithophila metallica in the Eurasian magpies (Pica pica) in Turkey. The study results provided valuable data on the ectoparasites living on migratory and resident bird species during their migration throughout northwest Turkey. Further research on the related pathogens that these ectoparasites harbor is in need.

Almost no systematic monitoring of bird population trends occurs in West Africa, despite rapid human population increase, habitat change, and climate change, making conservation planning problematic. We monitored bird population trends using constant-effort mist netting, in a newly protected area (Amurum Forest Reserve) on the outskirts of Jos, central Nigeria, from 2002 to 2019. We modelled the 18-year changes in trends of 10 Palearctic migrant and 41 common resident bird species and related this to any changes in annual environmental site quality using NDVI and rainfall data. The populations of most bird species were stable; 30% of migrants and 7% of residents increased, while 10% of migrants and 29% of residents declined moderately. Primary productivity, measured by NDVI, increased, and rainfall pattern was stable, suggesting that environmental conditions at the site improved slightly during the period. However, only a few species showed significant correlations of population trends with NDVI and rainfall. Overall, our results suggest that population changes were locally similar for both the Afro-Palearctic and resident bird species, being reasonably stable or increasing — although perhaps this reflected the fact that the monitoring was done within a newly protected area, which at present represents the best habitat in the wider locality. Those species that declined were mostly associated with open, grassland areas, which will have decreased as anthropogenic influences were reduced at the study site. Though we only monitored one site, the results are encouraging in that simple protection of a small habitat fragment (∼300 ha) in Nigeria yielded generally positive population benefits for both resident and Palearctic migrant species.

The latitudinal and elevational patterns of species richness of resident and migrant birds have been of interest to researchers over the past few decades, and various hypotheses have been proposed to explain the factors that may affect these patterns. This study aimed to shed light on the elevational distribution patterns of resident and migrant bird species richness by examining biotic and abiotic factors such as climate, and habitat heterogeneity using a piecewise structural equation model (pSEM). The overall pattern of resident species richness showed a decreasing trend with increasing elevation, whereas that of migrant species richness showed an increasing trend. The mid-peak pattern of species richness was affected by a combination of resident and migrant species and not by either resident or migrant species. Our results showed that resident species were distributed in lower elevation regions with higher mean spring temperatures, whereas migrant species were found in higher elevation regions with lower mean spring temperatures and higher overstory vegetation coverage. Although high elevation conditions might adversely affect the reproduction of migrant birds, higher overstory vegetation coverage at high elevations seemed to compensate for this by providing a better nesting and roosting environment. Despite the significance of habitat diversity and understory vegetation coverage in univariate linear regression models, multiple regression models of the interconnection of ecological processes demonstrated that mean spring temperature and overstory vegetation coverage were more explanatory than other variables.

Migratory birds are considered one of the main sources for West Nile virus (WNV) introduction into European countries. Following the WNV epidemic in the late summer of 1998 in a marshy area of Tuscany (Padule of Fucecchio), an extensive ornithological surveillance programme was carried out in the infected areas from 2006 to 2008. Several species of migratory and resident birds were trapped, sampled and serologically tested. The results of this surveillance programme gave a useful indication of potential sources of WNV re-introduction and spread into Italy. The area under study was also investigated and classified into ecological areas through satellite image processing. In August 2008, the WNV infection re-emerged in Italy in the area surrounding the Po river delta, involving three regions: Lombardy, Emilia Romagna and Veneto. Several surveillance activities were immediately put in place, including the extensive monitoring of wild birds found dead or trapped in the framework of other surveillance programmes. These activities were also prolonged in the 2009, when the virus circulation re-occurred at the border of the area already infected in 2008. The possible epidemiological role of the different species of migratory and resident birds is discussed, in relation to the different ecological patterns identified in the area and their potential ability to introduce, spread and support the endemization of WNV infection.

Prevalence and genetic diversity of avian haemosporidian parasites at an intersection point of bird migration routes: Sultan Marshes National Park, Turkey

Egypt has diverse weather throughout the year, which makes it attractive to many types of birds. So, the present study surveyed some of the resident and migratory bird species in Yousef El-Seddik district, Fayoum Governorate, Egypt, from 2018 to 2020. Birds were counted for four successive days monthly during the study period. The observed bird species were counted, identified, and classified. Also, the registered birds were divided into resident and migratory bird species. The results pointed out that there are 78 bird species belonging to 12 orders and 30 families that were recorded from 2018 to 2020. Also, the results revealed that seventeen birds were residents, while 59 birds were migratory. Finally, it was concluded that the order Passeriformes was the largest order registered in the study district, followed by Charadriiformes, followed by Ciconiiformes and Anseriformes, while the rest of the orders came in the last rank.

BackgroundWest Nile virus (WNV) and Usutu virus (USUV), both belonging to the genus Flavivirus, are emerging in Italy as important human and animal pathogens. Migratory birds are involved in the spread of Flaviviruses over long distances, particularly from Africa to Europe. Once introduced, these viruses can be further be dispersed by short-distance migratory and resident bird species. Thus far, there is still a considerable knowledge gap on the role played by different bird species in the ecology and transmission mechanisms of these viruses. The Region of Trentino-Alto Adige (north-eastern Italy) is located on the migratory route of many of the short- and long-distance migratory birds that cross the Alps, connecting northern Europe and western Asia with southern Europe and Africa. Until now, only a silent circulation of WNV and USUV within the territory of the Province of Trento has been confirmed by serological screening, whilst no cases of infected humans or animals have so far been reported. However, continuous spillover events of both viruses have been reported in neighbouring Regions. The aim of this study was to monitor the circulation of WNV and USUV in Trentino-Alto Adige, in order to detect if active virus shedding occurs in migratory birds captured during their seasonal movements and to evaluate the role that different bird species could play in the spreading of these viruses.MethodsWe carried out a biomolecular survey on oral and cloacal swabs collected from migratory birds during seasonal migrations. Birds belonging to 18 transaharian and 21 intrapaleartic species were examined during spring (n = 176) and autumn (n = 146), and were tested using a generic nested-PCR.ResultsAll samples tested negative for Flaviviruses. The possible causes of unapparent shedding, along with ecological and epidemiological implications are discussed.ConclusionsThe lack of detection of active virus shedding in these bird species does not exclude the circulation of these viruses within the Trentino-Alto Adige region, as reported in previous studies. The possible ecological implications are discussed.

The specificity of an animal's habitat requirements will determine its ability to deal with anthropogenic climate and habitat change. Migratory birds are thought to be particularly vulnerable to such change, but theory predicts that they should be largely generalists. This prediction was tested with the aim of assessing whether migratory Palaearctic‐breeding birds wintering in the savannah biome of Africa are more or less generalist in their habitat use compared with taxonomically and ecologically similar Afro‐tropical resident species. The degree of specialization of these species groups to certain habitat characteristics was assessed and compared by calculating the relative occurrence of the species along habitat gradients, where wide occurrence indicates generalism and narrow occurrence indicates specialism. Palaearctic migrants as a group could not clearly be distinguished as generalists relative to Afro‐tropical residents with respect to habitat attributes. The only indication of greater flexibility in Palaearctic migrants was a significant tendency to use habitats over a wider latitudinal range. The results suggest that migrants are generalists, but not necessarily more generalist than taxonomically similar resident species that also occur over a wide range of habitat types within the savannah biome. The availability of specific habitat requirements on the wintering grounds in Africa is therefore unlikely to be a primary limiting factor for many Afro‐Palaearctic migratory bird species.

Theobroma cacao plantings, when managed under the shade of rainforest trees, provide habitat for many resident and migratory bird species. We compared the bird diversity and community structure in organic cacao farms and nearby forest fragments throughout mainland Bocas del Toro, Panama. We used this dataset to ask the following questions: (1) How do bird communities using cacao habitat compare to communities of nearby forest fragments? (2) To what extent do Northern migratory birds use shaded cacao farms, and do communities of resident birds shift their abundances in cacao farms seasonally? (3) Do small scale changes in shade management of cacao farms affect bird diversity? Using fixed radius point counts and additional observations, we recorded 234 landbird species, with 102 species that were observed in both cacao and forest fragments, 86 species that were only observed in cacao farms, and 46 species that were restricted to forest fragments. Cacao farms were rich in canopy and edge species such as tanagers, flycatchers and migratory warblers, but understory insectivores were nearly absent from cacao farms. We observed 27 migratory species, with 18 species in cacao farms only, two species in forest only, and seven species that occurred in both habitats. In cacao farms, the diversity of birds was significantly greater where there was less intensive management of the canopy shade trees. Shade tree species richness was most important for explaining variance in bird diversity. Our study shows that shaded cacao farms in western Panama provide habitat for a wide variety of resident and migratory bird species. Considering current land use trends in the region, we suggest that action must be taken to prevent conversion away from shaded cacao farms to land uses with lower biodiversity conservation value.

Phylogenetic characterization of a reassortant H5N2 influenza A virus from a resident Mexican duck (Anas diazi)

BackgroundThe range of vertebrate hosts on which species of mosquito blood-feed is an important parameter for identifying potential vectors and in assessing the risk of incursion and establishment of vector-borne pathogens. In the United Kingdom, studies of mosquito host range have collected relatively few specimens and used techniques that could only broadly identify host species. This study conducted intensive collection and analysis of mosquitoes from a grazing marsh environment in southeast England. This site provides extensive wetland habitat for resident and migratory birds and has abundant human nuisance biting mosquitoes. The aim was to identify the blood-feeding patterns of mosquito species present at the site which could contribute to the transmission of pathogens.MethodsTwice-weekly collections of mosquitoes were made from Elmley Nature Reserve, Kent, between June and October 2014. Mosquitoes were collected using resting boxes, by aspiration from man-made structures and using a Mosquito Magnet Pro baited with 1-octen-3-ol. Blood-fed specimens were classified according to the degree of blood meal digestion using the Sella scale and vertebrate origin determined using sequencing of a fragment of the mitochondrial cytochrome C oxidase subunit I gene. Mosquitoes that were morphologically cryptic were identified to species level using multiplex PCR and sequencing methods.ResultsA total of 20,666 mosquitoes of 11 species were collected, and 2,159 (10.4%) were blood-fed (Sella scale II-VI); of these 1,341 blood-fed specimens were selected for blood meal analysis. Vertebrate origin was successfully identified in 964 specimens (72%). Collections of blood-fed individuals were dominated by Anopheles maculipennis complex (73.5%), Culiseta annulata (21.2%) and Culex pipiens form pipiens (10.4%). Nineteen vertebrate hosts comprising five mammals and 14 birds were identified as hosts for mosquitoes, including two migratory bird species. Feeding on birds by Culex modestus and Anopheles atroparvus populations in England was demonstrated.ConclusionsThis study expands the vertebrate host range of mosquitoes in the Thames estuary region of the UK. Feeding on both resident and migratory bird species by potential arbovirus vectors including Cx. pipiens f. pipiens and Cx. modestus indicates the potential for enzootic transmission of an introduced arbovirus between migratory and local bird species by native mosquito species.

The prevalence of , , and haemosporidian infections was investigated at three stations of the Khingan Nature Reserve, Amur Region, Russian Far East. Between 2021 and 2024, we sampled mainly passerine birds, with a total of 751 individuals belonging to 68 species. Birds were mist-netted during the breeding season at the "Kleshenskii" and "Karapcha" stations, and during the spring and autumn migrations at the "Lebedinii" station. The prevalence of different haemosporidian genera varied between "Karapcha" (coniferous–deciduous forest) and "Kleshenskii" (swampy plain with forest patches): and parasites were significantly more common at the "Karapcha" station, while parasites dominated at the "Kleshenskii" station. Based on these data and on infection patterns in resident bird species, we hypothesize that haemosporidians from all three genera can be transmitted at "Karapcha", but only (or mainly) at "Kleshenskii". Long-distance migrants had a higher prevalence of than sedentary bird species at both "Kleshenskii" and "Karapcha". We assume that parasites are also actively transmitted on the wintering grounds. At the "Lebedinii" station, the frequency of infections was higher in autumn than in spring. This pattern could be related to parasite transmission in the northern latitudes where the birds migrating through "Lebedinii" breed. In general, birds can become infected by haemosporidians either (1) on the breeding grounds or (2) on the wintering grounds and during migration. Our study revealed that, among breeding birds, location (i.e. "Kleshenskii" vs. "Karapcha") most strongly affected the infection prevalence, whereas among migrants at "Lebedinii", season (spring vs autumn) was the key factor. Whether a species was resident or migratory rendered a much smaller effect. We thus conclude that, in our study area, the prevalence of haemosporidian infection depended more on breeding-ground conditions than on those at the wintering grounds.

Landscape structure and configuration may affect bird body condition, with contrasting effects on resident and migratory species. There is little empirical evidence to support this hypothesis in tropical regions, where land-use change poses a major threat to biodiversity. We aimed to assess the effects of landscape structure and configuration on the body condition of neotropical migrant and resident bird species. We compiled body condition data (using the scaled mass index) of nine bird species (five resident and four migratory). We characterized landscape structure and configuration at 26 localities. We evaluated the effects of landscape metrics on bird body condition using Bayesian linear mixed models. The landscapes in our study largely varied in forest, crop, and grassland cover, as well as in landscape metrics. When we examined migrant birds, we found a positive effect of landscape connectivity and crop cover on body condition. Similarly, body condition of resident birds was positively affected by connectivity and crop cover, but also by forest patch area and capture day. Changes in landscape structure and configuration may indirectly alter the access to resources, causing additional energy expenditures, leading to a deteriorated body condition. Conversely, landscape heterogeneity may have a positive effect on bird body condition. Therefore, we recommend maintaining connectivity and complementary resources in the landscape.