Abstract
<p class="abstrak3">Avian Beta Defensin 2 (AvBD2) gene, which is located in chromosome 3, plays an important role in the immune system of the chicken by inhibiting the development of microorganisms such as bacteria that infect body tissues. Defensins are produced through epithelial cells immediately after tissue injury or infection, which then processes the maturation of dendritic cells to initiate an immune response in the lymph nodes. The purpose of this study was to discover the polymorphism of the AvBD2 gene in IPB-D1 chickens. PCR and direct-DNA sequencing methods were used to identify the diversity of intron 1, exon 2, and intron 2 AvDB2 genes in 47 chickens. Genotype and allele frequency, and heterozygosity calculations were carried out to obtain information of the AvBD2 gene polymorphism. A total of 10 single nucleotide polymorphisms were found in the AvBD2 gene located in intron 1 (g.4843T&gt;A, g.4853G&gt;A, and g.4859T&gt;C), exon 2 (g.4881A&gt;G, g.4889G&gt;A, and g.5002C&gt;T), and intron 2 (g.5075C&gt;T, g.5111T&gt;G, g.5116G&gt;T, and g.5177G&gt;T). All SNPs are polymorphic. The g.5002C&gt;T mutation causes changes in the amino acid Ala to Val which has the potential to be a candidate for characterizing disease resistance in IPB-D1 chickens.</p>
Highlights
Indonesia's native chicken population in 2020 is estimated to reach 308 million chickens, or around 8.8% of the total national chicken population (DGAHP 2020)
The objective of this study is to discover the Single Nucleotide Polymorphism (SNP) of the partial Avian Beta Defensin 2 (AvBD2) gene in IPB-D1 chicken using direct-DNA sequencing method
A total of 10 single nucleotide polymorphysms (SNP) were found in intron 1 (3 SNPs), exon 2 (3 SNPs), and intron 2 (4 SNPs) of the AvBD2 gene (Table 1)
Summary
Indonesia's native chicken population in 2020 is estimated to reach 308 million chickens, or around 8.8% of the total national chicken population (DGAHP 2020). Application of extensive traditional rearing system shows the low application of biosecurity and sanitation. The lack of attention of local chicken breeders. *all authors contributed to the work as main contributors breeders to good rearing management and the application of biosecurity affect the health of local chickens. This condition causes local chicken production to be not optimal. Availability of local chicken breeds that are resistant to disease is very important so that breeders could get disease-resistant chickens maintained in suboptimal biosecurity and sanitation conditions. One of the efforts to provide these breeds is through genetic selection of local chickens that have a good immune system
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