Abstract
Polyploidy has many advantages over diploidy, such as rapid growth, sterility, and disease resistance, and has been extensively applied in agriculture and aquaculture. Though generation of new polyploids via polyploidization has been achieved in plants by different ways, it is comparatively rare in animals. In this article, by a chemical compound, SP600125, polyploidization is induced in fish cells in vitro, and a stable autotetraploid cell line has been generated from diploid fibroblast cells of crucian carp. As a c-Jun N-terminal kinase (Jnk) inhibitor, SP600125 does not function during the induction process of polyploidization. Instead, the p53 signal pathway might be involved. Using the SP600125-induced tetraploid cells and eggs of crucian carp as the donors and recipients, respectively, nuclear transplantation was conducted such that tetraploid embryos were obtained. It suggests that combining polyploidization and the somatic cell nuclear transfer technique (SCNT) is an efficient way to generate polyploidy, and the presented method in this research for generating the tetraploid fish from diploid fish can provide a useful platform for polyploid breeding.
Highlights
Polyploidy is useful in breeding new plant and animal species
Karyotype analysis showed that the majority of cells were normal diploid (90%, 2N = 100) (Fig. 1C). These results indicate that the fin cell line was a stable diploid crucian carp cell line
We found that Jun N-terminal kinase (Jnk) levels were higher in the normal diploid cells than in tetraploid cells, especially Jnk[1]
Summary
Polyploidy is useful in breeding new plant and animal species. Because it has more than two sets of chromosomes, the polyploid has some advantages over the diploid, such as rapid growth, sterility, and disease resistance. Several induction methods have been reported to generate polyploidy. A new method combining the polyploid cell generation and SCNT to generate stable polyploid animals seems promising[6,7,8,9,10]. New chemical compounds that induce artificial polyploidy in mammalian cells have been screened[5], though the generation of polyploid cells in fish remains difficult. No stable polyploid fish cell lines generated by chemical induction have been reported. Analysis of DNA content of cells collected from 4n and 8n peaks
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