Abstract
An automated protein purification approach was developed on a centrifugal platform. The major fluidic functions include sequential reagent addition, drainage, and manipulation of magnetic beads. The sequential reagent addition is performed by an Euler-force-induced decanting approach and the reagent drainage is conducted through a siphoning method. A magnetic module was designed to maintain the magnetic beads in a dispersed state while immobilizing them in the reaction chamber during reagent drainage. Finally, an automated protein purification platform, that is able to complete the purification process within an hour, was developed.
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