Abstract
Due to the unstable nature of rifampicin, a rapid automated high-performance liquid chromatographic method had to be developed for the analysis of a large number of plasma samples generated during a bioavailability trial. Extraction and injection of the samples were automatically done by a sample preparation system using C 2, 100 mg Bond Elut extraction columns. The extracts were chromatographed on a 4-μm reversed-phase C 18 column with a citrate buffer and acetonitrile as mobile phase. The analytes were detected at 342 nm. Calibration curves were linear to at least 20 μg/ml and the limit of quantification was 0.16 μ/ml.
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