Audiogenic seizure severity and hearing deficits in the genetically epilepsy-prone rat

Abstract

Hearing deficits have been observed in rodents that are susceptible to audiogenic seizures (AGS), including the genetically epilepsy-prone rat (GEPR). AGS sus-ceptibility can be induced in normal animals by treatments that damage the cochlea. In this study, we measured the relative degree of hearing loss in animals from the GEPR substrains that exhibit different degrees of AGS severity and examined the relationship between the deficit and the AGS severity. Auditory brain stem response (ABR) thresholds to clicks in the GEPR substrain that exhibits exclusively maximal AGS severity (GEPR-9) were significantly elevated, and latencies for ABR peaks I, III, and IV were significantly increased as compared to normal Sprague-Dawley rats. ABR thresholds for the substrain of GEPRs that exhibit exclusively submaximal AGS (GEPR-3) were even higher than those in the GERP-9, and ABR waveforms were distorted. ABR peak IV was significantly longer than normal in the GEPR-3 substrain, as were mean interpeak intervals and central conduction times. These data indicate that significant hearing deficits occur in the GEPR-3 substrain. In non-AGS-susceptible progeny of the GEPR-9 [GEPR-0(9)], ABR thresholds were not significantly different from normal. These data along with studies of ABR thresholds in thyroid-deficient rats suggest that an inverted Ushaped relationship exists between hearing deficit and AGS severity. That is, moderate threshold elevations are associated with increasing AGS severity, but when the hearing deficit exceeds a certain level, a decrement in AGS severity occurs.