Attenuating potential of some antioxidants: Cellgevity, max one, purslane and vitamin C on caffeine induced hormonal and testicular toxicities in male albino rats

Abstract

Background: Infertility challenges in men, resulting from disturbances in hormonal balance and testicular integrity, stands as a significant health challenge associated with various factors. Consequently, diverse strategies are necessary to tackle this issue. This research explored the attenuating potentials of some antioxidants—Cellgevity (CG), Max One (MX), purslane, and vitamin C (VC)—on caffeine-induced hormonal and testicular toxicities in male albino rats. Methodology: Sixty sexually matured male albino rats were randomly divided into ten groups consisting of two rats in three replicates using completely randomized design (CRD). Group one served as control and received water and feed only. Group two were given 200 mg/kgBw of CG, group three received 200 mg/kgBW of MX, group four received 100 mg/kgBW of VC, group five received 200 mg/kgBW of caffeine, group six received 200mg/kgBW of purslane, group seven received 200 mg/kgBW of caffeine and 200 mg/kgBW of CG, group eight received 200 mg/kgBW of caffeine and 200 mg/kgBW of MX, group nine received 200mg/kgBW of caffeine and 200 mg/kgBW of purslane, group ten received 200 mg/kgBW of caffeine and 100 mg/kgBW of VC. Administration was done orally and lasted for 65days. The rats were sacrificed after administration using chloroform anaesthesia. The testes were processed for histology while blood sample were obtained for hormonal assay. Results: The results showed that caffeine significantly (p<0.05) reduced the serum levels of testosterone, follicle stimulating hormone (FSH), luteinizing hormone (LH) and estradiol when compared to the control and other treatments groups. There was testicular toxicity with loosely packed enlarged seminiferous tubules in caffeine treated animals when compared with the control and antioxidants treated animals. However, CG, MX, purslane and VC attenuated the effect of caffeine in all the parameters evaluated by increasing the levels of the hormones and restoring testicular integrity of the animals in the combination groups. Conclusion: This present study has revealed the toxic effect of caffeine reproductive hormones and testicular integrity of male albino rats. However, the findings of this study provided substantial evidence on the attenuating effects of CG MX, purslane and VC on caffeine-induced hormonal and testicular toxicity in male rats as mammalian models.