Abstract
Fourteen of 15 human sera displayed inhibition of the characteristic haemagglutination (HA) of oral strains of Fusobacterium nucleatum in a microtitre haemagglutination inhibition (HI) test. Chromatography of a gamma globulin pool revealed the activity in the fractions eluting with IgM and IgA; absorption procedures with anti-human μ and α chain sera did not remove the activity. Complete saturation of the serum with (NH 4) 2SO 4 produced a floating mucinous fraction displaying HI activity. Sepharose 4B Chromatography of glycoprotein preparations gave mol. wt in excess of 400,000; antibody was not present. Glycoprotein from sera of various blood types aggregated whole F. nucleatum cells. EDTA and galactose inhibited this aggregation whereas glucose did not inhibit. Thus serum glycoproteins containing galactose probably bind to the surface of the F. nucleatum and this binding may influence colonization in the gingival sulcus.
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