Abstract
This study presents, attenuated total reflection Fourier transforms infrared spectroscopy of dried serum samples in an effort to assess biochemical changes induced by non-Hodgkin’s lymphoma and subcutaneous melanoma. An EL4 mouse model of non-Hodgkin lymphoma and a B16 mouse model of subcutaneous melanoma are used to extract a snapshot of tumor-associated alteration in the serum. The study of both cancer-bearing mouse models in wild types and their corresponding control types, emphasizes the diagnostic potential of this approach as a screening technique for non-Hodgkin lymphoma and melanoma skin cancer. Infrared absorbance values of the different spectral bands, hierarchical clustering and integral values of the component bands by curve fitting, show statistically significant differences (student’s t-test, two-tailed unequal variance p-value < 0.05) between spectra representing healthy and tumorous mouse. This technique may thus be useful for having individualized route maps for rapid evaluation of lymphoma and melanoma status and associated therapeutic modalities.
Highlights
The incidence rates of cutaneous melanoma[1], a deadly form of skin cancer, has been increasing in many regions and populations over the last few decades[2]
The Fourier Transform Infrared (FTIR) spectroscopy combined with appropriate data handling frameworks has been widely applied in many oncological studies[9] such as studies involving the cancers of the cervix[13], the lung[14], the breast[15], the skin[16], the gastro-intestine[17], the prostate[18], the colon[19], the ovary[20], the urinary bladder[21] and many other body parts
We demonstrate the diagnostic capability of Attenuated Total Reflection (ATR)-FTIR spectroscopy for the melanoma and non-Hodgkin’s lymphoma (NHL) by testing air-dried serum samples from respective mouse models
Summary
The incidence rates of cutaneous melanoma[1], a deadly form of skin cancer, has been increasing in many regions and populations over the last few decades[2]. The FTIR spectroscopy combined with appropriate data handling frameworks has been widely applied in many oncological studies[9] such as studies involving the cancers of the cervix[13], the lung[14], the breast[15], the skin[16], the gastro-intestine[17], the prostate[18], the colon[19], the ovary[20], the urinary bladder[21] and many other body parts. We demonstrate the diagnostic capability of ATR-FTIR spectroscopy for the melanoma and NHL by testing air-dried serum samples from respective mouse models
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
