Abstract
Esterases and lipases originating from the metagenome (environmental DNA) were studied for the asymmetric hydrolysis of cis-3,5-diacetoxycyclopent-1-ene 1. Out of 83 enzymes, 35 were found to be active. Subsequent analytical and laboratory scale reactions identified three enzymes showing excellent (−)-preference and one enzyme with excellent (+)-selectivity exceeding even the performance of recombinant or commercial pig liver esterase, which is known as the best biocatalyst for this compound.
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