Abstract
During a survey on mustard fields in 2016, symptoms including flat stem, phyllody and virescence were observed at Jhangha (Gorakhpur district) and Kumarganj (Faizabad district), Uttar Pradesh, India. The total DNA extracted from symptomatic mustard samples from both locations (GKP and FZ samples) yielded 1.3 kb amplicons with the universal phytoplasma primers P1/P7 followed by 3Far/3Rev in nested PCR assays. Samples from asymptomatic mustard plants from both locations resulted negative in the same assays. BLAST analysis of 16S rRNA partial gene sequence of one mustard GKP strain showed sequence similarity with phytoplasmas classified in rice yellow dwarf group (16SrXI), while the mustard phytoplasma FZ strain showed similarity with strains belonging to the clover proliferation (16SrVI) group. Phylogenetic analysis and virtual gel plotting on 16S rDNA sequences of both mustard phytoplasma strains indicated their closer relationships with 16SrXI-B and 16SrVI-D phytoplasma subgroups, respectively. This is the first detection and preliminary identification of these phytoplasmas in mustard.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
