Abstract
Objectives: Several candidate genes are implicated in the pathogenesis of type 2 diabetes mellitus (T2DM), one of which is interleukin (IL-10). In this investigation, we aimed to study the association between IL-10 (-592A/C) gene polymorphism with its level in T2DM with and without nephropathy. Methods: IL-10 (-592A/C) gene polymorphism was genotyped using restriction fragment length polymorphism (RFLP-PCR) technique and IL-10 levels were measured in two groups of T2DM, one group complicated with nephropathy and the second non-complicated. Results: Our results revealed no significant differences in IL-10 (−592A/C) genotypes distribution between the two groups of T2DM patients. IL-10 levels were found significantly elevated in diabetic nephropathy patients compared to T2DM without nephropathy (P<0.05) and were positively correlated to the degree of albuminuria (r=0.61, P<0.01). IL-10 levels increased in IL-10- (−592C/C) genotype compared to IL-10-(−592A/A) and IL-10- (−592A/C) genotypes in diabetic nephropathy patients while such difference was not found in T2DM patients without nephropathy. Conclusion: IL-10 (-592A/C) gene polymorphism and IL-10 level play a role in the pathogenesis of nephropathy in T2DM.
Highlights
Type 2 diabetes mellitus (T2DM) is one of the main chronic diseases and its complications have become a major cause of morbidity, mortality, and disability in the World [1]
We aimed to study the association of IL-10 (−592A/C) gene polymorphism with IL-10 level in T2DM with and without Diabetic nephropathy (DN)
Genotyping IL10-592 A/C single nucleotide polymorphisms were genotyped by restriction fragment length polymorphism (RFLP) according to the method of Mori et al [11]
Summary
Type 2 diabetes mellitus (T2DM) is one of the main chronic diseases and its complications have become a major cause of morbidity, mortality, and disability in the World [1]. IL-10, an 18-kDa glycoprotein plays a key role in homeostatic control of inflammatory and immune responses. It is produced by T-helper 2 (Th2) cells and a wide variety of cell types including B cells, monocytes, macrophages, keratinocytes, and many tumor cells [5]. IL-10 polymorphic variants are linked with cytokine production and are involved in many chronic inflammatory diseases, including T2DM [9]. In this investigation, we aimed to study the association of IL-10 (−592A/C) gene polymorphism with IL-10 level in T2DM with and without DN
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