Association of Interleukin 6 (IL6) and 8(IL8) among Women with Spontaneous Abortion in Khartoum State 2018

Abstract

Background: Interleukin 6 and Inetrleukin 8 are secreted by macrophage and have been shown to exert deleterious effects on pregnancy, inhibiting fetal growth and development. Possible immunologic causes of spontaneous miscarriages have been extensively researched. The change in the cytokines balance synthesis in favor of those synthesized by macrophage cells with an increase of interleukin 6 and interleukin8 secretion is considered essential for maintaining a normal pregnancy. Aim: To determine the level of cytokines (interleukin6 and interleukin8 ) in pregnant and aborted women to assess the role of cytokines (IL 6 and IL8 ) as a factor to predict the outcome of pregnancy and To compare between serum interleukin 6 and 8 in women with spontaneous abortion and pregnant women. Method: This across sectional study tested using Five ml of venus blood were collected from patients with abortion and healthy pregnant and non-pregnant control in plain container. All samples were centrifugated at 3000 rpm for 5 minutes and seperated sera were collected in sterile tubes. Serum was treated according to uniform standards and stored at – 20 refrigerators until analysed. Serum IL 6 and IL8 were determined by Enzyme Linked Immunosorbent Assay (ELISA) .All samples were run according to manufacturer instructions (Human IL, Bio legend, USA) with linearity range 0.94- 60.0 pg/ml. Results: There is a highly significant increase of control of pregnant women (P<0.01) in concentration of IL6 (6.8 ±5.1)pg/ml in compare to aborted women in different trimesters of pregnancy. Whereas healthy control non pregnant women show highly significant decrease in concentration (1.6 ±0.65)pg/ml compared to abortion patients. The results of this study showed there was a highly significant increased (P<0.01) concentration of IL-8 (26.5 ±13.1) pg/ml in serum of aborted women in trimesters of pregnancy,compared with control group of non-pregnant women which were(6.9 ±0.93) while control group of pregnant women show slightly significance increased(P<0.01) concentration (31.1 ±14.1)pg/ml compared with aborted women. Conclusion: The serum level of interleukin 6 and 8 was significantly higher in pregnant women as compared to aborted women .The serum level of interleukin6 and 8 in aborted women was significantly higher as compared to non-pregnant women. The results indicated there is no clarify association between cytokines (IL6 and IL8) and abortion.