Abstract
The potential of the chlorophyll fluoresence technique in screening for frost sensitivity in seedling progenies representing 18 genotypes of wild and cultivated potato from different geographical and altitudinal origins was assessed by measuring the decrease in variable chlorophyll fluorescence (Fvar) of terminal leaflets of hardened and non-hardened plants after freezing at −5°C for 60 min and subsequent re-warming to 20°C. The method was rapid and the results obtained agreed well with a visual assessment of frost injury carried out after leaflets had been returned to optimal growth conditions for 72 hours.Solanum tuberosum CPC 3294 originating from Chile was the most frost-sensitive genotype studied andSolanum albicans CPC 3712 from high-altitude regions of Peru, the most frost-resistant. Frost damage increased with length of exposure to frost and this was associated with a reduction of variable fluorescence and quenching capacity of the thylakoid membranes.
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