Assessment of the Allelopathic Potential of Juniperus ashei on Germination and Growth of Bouteloua curtipendula

Abstract

Potential allelopathic compounds of Juniperus ashei Buchh. (Ashe juniper) and their effect on a native grass were determined in laboratory and field studies. Solid-phase microextraction and gas chromatography/mass spectrometry were used to determine if monoterpenes found in the essential oils of J. ashei are released in leaf and litter leachate, as well as volatilized from leaf tissue. Camphor, bornyl acetate, and limonene were found in leaf and fresh litter leachates; however, degraded litter did not contain any of these compounds. Camphor was the most common potentially allelopathic compound found in J. ashei leaf and litter leachate and in volatiles from leaf tissue. The effects of leaf and litter tissue on the germination of Bouteloua curtipendula (Michx.) Torr. (side-oats grama) was tested by using the "sandwich agar method". The highest germination of B. curtipendula (29.6%) occurred in the control, which was significantly higher than fresh litter (13.2%) and degraded litter (16.2%). The lowest germination (6.2%) occurred with J. ashei leaves. In the field experiment, aboveground dry mass of B. curtipendula was evaluated in relation to position within the canopy and intercanopy of J. ashei adult trees when light and water were held constant across locations. Aboveground dry mass of B. curtipendula was significantly greater in the intercanopies of J. ashei (163.7 g m(2)) compared to the dry mass in the understory (44.8 g m(2)) and dripline (44.5 g m(2)), suggesting some negative influence by J. ashei. Chemical analyses indicate that monoterpenes are released through leaching and volatilization from J. ashei, and germination and field studies suggest that these compounds inhibit B. curtipendula.