Abstract
The natural phenotypic variations present in the cultivated plants can be linked to the molecular polymorphism by association genetics. The present study was aimed to screen the drought tolerant genotypes by morphological, biochemical and measuring the genetic diversity by using Simple Sequence Repeats (SSR) markers. An experiment consisting of 26 genotypes was conducted in the laboratory of Biotechnology University of Azad Jammu and Kashmir Muzaffarabad, Pakistan. Seeds were grown in pots and 4 weeks older leaves were used for DNA extraction. Twenty-six genotypes of Tomato (Lycopersicon esculentum Mill.) were fingerprinted with 30 (SSR) markers by using Polymerase Chain Reaction (PCR) technique. Nei’s genetic distances for SSR markers, data was calculated and relation matrix between genotypes was shown graphically in the form of a dendrogram. All the 26 tomato genotypes showed genetic distances of 1.0 - 2.20 between them. A smallest genetic distance 0.2 was recorded between genotypes G6-17909, G5-17904 and G21-006234, G12-0852, G7- 88572 G32 -19233, G28-17903, G12-17806 indicating closer relationship between the genotypes. The most distant accessions were G7-1059 and G45-19212. The marker (SSR) based fingerprints will assist for their future potential in crop improvement. Keywords: Analysis; Cluster; Diversity; Genetic; SSR; Tomatoes http://dx.doi.org/10.19045/bspab.2020.90160
Highlights
The molecular markers such as amplified fragment length polymorphism (AFLP), random amplified polymorphic DNA (RFLP), random amplified polymorphic DNA (RAPD), single nucleotide polymorphism (SNP), and simple sequence repeats (SSR) can be effectively used for a variety identification because they are independent of environmental factors [6, 7]
All the 26 tomato genotypes were grouped into 2 main clusters and 4 sub clusters
The morphological attributes provide a simple technique for quantification of genetic variation by assessing the performance under different environmental conditions [20, 21]
Summary
Twenty-six genotypes of Tomato (Lycopersicon esculentum Mill.) were fingerprinted with 30 (SSR) markers by using Polymerase Chain Reaction (PCR) technique. The tomato genetic was started from Latin America, it is the materials are important resources for second most significant vegetable crop breeding purposes and biotechnology and cultivated all over the world. It gives both their relationship studies have potential value pro-vitamin A and vitamin C to the human in tomato industry. The DNA molecular markers can be used to study the genetic diversity and the variations in the genus Solanum and for selecting the tomato [1, 9-15]. Simple sequence repeats (SSRs), are recognized as microsatellites, bear good results in molecular research because of the properties like having high reproducibility, G7-10593, G11-17895
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