Abstract
In the present study, ten genotypes of Lablab purpureus L. Sweet bean were screened for genetic divergence by using 5 primers, i.e., OPA 01, OPA 04, OPA 13, OPB 09, and OPB 10. Analysis of diversity such as PIC, MI, MRP, Dendrogram, and RP was done. Out of the 5 primers used, the average number of Polymorphic bands detected was 7.6 and the average level of polymorphism was 74.23%. OPA 01 showed the highest level of polymorphism (92.85%) while OPB-09 showed the least level of polymorphism, i.e., 55.55%. The polymorphic information content ranged from 0.24 (OPA-04) to 0.318 (OPA-01) at an average PIC value of 0.275. The mean of resolving power per RAPD primer is 3.65. The MI value ranged from 1.32 to 2.79 with a mean of 1.79/primer. The mean of MRP per primer was 0.47. The clustering of genotype was performed following the UPGMA method. The optimal tree with the sum of length branch is 13.30325.
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