Abstract
A quantitative categorization of total storage proteins profile of 6 isolates of Aspergillus flavus was performed by sodium dodecyl sulphate- polyacrylamide gel electrophoresis (SDS-PAGE). This technique was used to explore the level of genetic discrepancy in A . flavus isolates . Total soluble proteins were resolved on 10% resolving gel. A total of 27 polypeptide bands were obtained among which 20 bands were present in all isolates but other 7 bands of molecular weight (127.38, 110.14, 109.74, 97.62, 93.83, 74.98 and 59.03 KDa) showed variation. Dendrogram based on electrophoretic data clustered the varieties mainly into two groups. In conclusion, electrophoretic bands (SDS-PAGE) of storage proteins can provide a potent tool to estimate genetic variation and relation among isolates. The specific bands of seed storage protein profiles may be used as markers for identification of the varieties.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
