Abstract
Cardiovascular fibrosis resulted from pressure overload or ischemia could alter myocardial stiffness and lead to ventricular dysfunction. Fluorescently labeled collagen-binding protein CNA 35, derived from the surface component of Staphylococcus aureus, and a novel synthetic biphenylalanine containing peptide are applied to stain fibrosis associated collagen and myocytes, respectively. Detailed pathological characteristics of cardiovascular fibrosis could be identified clearly in 2 hours. This staining pair requires only simple staining and brief washing, generating less than 10 ml of waste. The image information collected by this novel fluorescent staining pair is compatible with it collected by the traditional Masson's Trichrome and Picrosirius Red staining which are widely used to stain cardiovascular fibrosis and isolated cells.
Highlights
To assess the dynamic status of fibrosis, which is an alternation of myocardial collagen network, proper reagents that recognize collagen fibers in vitro and in vivo would be extremely valuable in understanding the definitive mechanism of hypertrophy and heart failure
We demonstrated the usage of CNA35 and myocyte targeting peptide (MTP) probes in assessing myocardial fibrosis induced by aortic constriction following coronary arterial occlusion and aorta debanding
The myocardium is an elastic network of myocytes enmeshed in a collagen matrix that connects the myocytes and supporting coronary vasculature
Summary
Fibrosis in the heart is associated with many cardiac diseases, such as hypertrophy and coronary ischemic heart disease.[1,2] To assess the dynamic status of fibrosis, which is an alternation of myocardial collagen network, proper reagents that recognize collagen fibers in vitro and in vivo would be extremely valuable in understanding the definitive mechanism of hypertrophy and heart failure. Histological analysis of heart tissues is the most accepted and sensitive way to study pathological changes of fibrosis. Masson’s Trichrome and Picrosirius Red are two commonly used collagen staining methods for cardiovascular fibrosis detection.[3,4] They provide clear contrast to distinguish collegens from surrounding connective tissue and cells. These methods are excellent in examining collagen density and structure, the procedure is lengthy and consumes large amount of reagents and washing solutions. Cleaner and more efficient histopathological stains, which could offer compatible histological details while conserving reagents, resource and time, would certainly be a favorable choice
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