ASSESSING THE RELATIONSHIPS OF SOME COCCOID GREEN LICHEN ALGAE AND THE MICROTHAMNIALES (CHLOROPHYTA) WITH 18S RIBOSOMAL RNA GENE SEQUENCE COMPARISONS1

Abstract

ABSTRACTEight complete nuclear‐encoded small‐subunit ribosomal RNA (18S rRNA) gene sequences were determined for four genera of the Microthamniales (Pleurastrophyceae) and forGloeotilopsis planctonicaIyengar & Philipose (Ulvophyceae, Ulotrichales) to investigate evolutionary relationships within the Microthamniales and the taxonomic position of this order within the green algae. Phylogenies inferred from these data revealed specific relationships at the level of genera and species that disagree with those inferred from vegetative cell morphology but agree with those inferred from motile cell characters. The rRNA phylogenies provide even better resolution than that gained from morphology alone. The coccoid lichen algaTrebouxiaspp. is specifically related to other coccoid lichen and soil algae (i.e.Myrmecia biatorellaeBoye‐Petersen andFriedmannia israelensisChantanachat & Bold), forming the “Lichen Algae Group,” an evolutionarily distinct lineage within the Microthamniales.Trebouxiais a paraphyletic andPleurastruma polyphyletic genus in rRNA phylogenies. In contrast to previous hypotheses based on morphology,Pleurastrum terrestreFritsch & John is not closely related toTrebouxiabut occupies an isolated position within the Microthamniales. The filamentous algaMicrothamnion kuetzingianumis not ancestral to coccoid members of the Microthamniales but is closely related with the coccoidFusochloris perforata(Lee & Bold) Floyd, Watanabe & Deason. The Microthamniales are inferred to be an array of independent lineages that radiate nearly simultaneously and may also include some autosporic coccoid taxa previously classified in the Chlorophyceae. Monophyly of the Microthamniales could not be demonstrated unequivocally. In contrast to a hypothesis based on ultrastructure, the Microthamniales are evolutionarily distinct from the Ulotrichales. The latter are ancestral to the radiation of the Microthamniales and the Chlorophyceae in the rRNA phylogenies.