Abstract
Applicability of triolein-embedded cellulose acetate membrane (TECAM) to accumulation and potential biological effect assessment for hydrophobic organic contaminants (HOCs) was investigated compared with Japanese medaka. The results of field exposure showed that medaka and TECAMs accumulated contaminants in a similar pattern with good correlations between concentrations in medaka and TECAMs based on lipid weight for OCPs (r=0.96, p=0.01, n=9) and PAHs (r=0.73, p=0.01, n=13). Meanwhile, 2,3,7,8-TCDD equivalents (TEQs) of TECAM extracts detected by in vitro H4IIE cell bioassay corresponded well to hepatic EROD activities of exposed fish and TEQs of water samples. We concluded that TECAM could be utilized as a surrogate for biomonitoring organisms to assess the bioaccumulation of HOCs and potential biological effect.
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