Abstract

Aspegillus niger 세포에 의한 구연산 생산에 대한 발효 매체로서 우유공장폐수가 연구되었다. 발효매체에 <TEX>$Mn^{2+},\;Fe^{2+}\;and\;Cu^{2+}$</TEX>의 첨가가 구연산 생산을 일정하게 증가시켰다. 그러나 다른 금속이온인 <TEX>$Mg^{2+}$</TEX>의 첨가는 구연산 생산을 감소시켰다. 구연산의 농도는 우유공장폐수에서 환원당 농도의 농도가 50 g/l와 100 g/1로 Aspegillus niger ATCC 9142에 의한 batch bioreactor에서 각각 7.2 g/1과 16.5 g/l로 높게 표시되었다. 본 실험에서 적용한 수리적 모델식들이 세포성장의 예상, 구연산 생산, 기질소모 속도에 대해 잘 적용되는지를 알아보기 위해서 발효반응 모델식에 적용시켜 본 결과 실험치와 잘 부합되었다. The waste water from a milk factory was investigated for possibility of use to the production of citric acid by cells of Aspegillus niger ATCC 9142. The addition of <TEX>$Mn^{2+},\;Fe^{2+}\;and\;Cu^{2+}$</TEX> ions to waste increased citric acid production steadily, but addition of metal ion <TEX>$Mg^{2+}$</TEX> decreased the citric acid production. The amount of produced citric acid by Aspegillus niger ATCC 9142 with addition 50 g/1 and 100 g/1 of reducing sugar in milk factory waste water were 7.2 g/1 and 16.5 g/1 respectively. Mathematical model was simulated for their predictability of cell growth, citric acid production and substrate consumption rate and coincided with experimental data.

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