Abstract

As(III) oxidation by a pure bacterial culture, Alcaligenes faecalis strain O1201, was evaluated using a continuous stirred tank reactor. The bioreactor system was operated for 204 days under a wide range of influent As(III) concentrations (500–8,000 mg∕L) and hydraulic retention times (25.6–90.7 h) with citrate as the sole carbon and energy source at pH 7 and 30°C. Nine steady-state conditions were obtained with As(III) oxidation efficiency exceeding 95%. The system quickly recovered from an As(III) overloading at 15,000 mg∕day∕L while it operated at a HRT of 12.8 h . A material balance analysis revealed that nearly all the As(III) removed in the continuous stirred tank reactor (CSTR) was oxidized to As(V) and only 3.2% of the As(III) fed to the CSTR was unaccounted for. Biokinetic parameters, kmc =0.86 mg∕mg cell dry weight/h and Kc =70 mg∕L , for As(III) oxidation were obtained using the Monod expression and data obtained at steady-state conditions.

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