Armillaria species of the Olympic Peninsula of Washington state, including confirmation of North American biological species XI

Abstract

Forty-eight collections of Armillaria from the Olympic Peninsula of Washington State were identified to species using the conventional pairing protocol and/or a polymerase chain reaction-based (PCR) technique utilizing restriction fragment poly? morphisms (RFLPs) of the intergenic spacer region (IGS) of the ribosomal DNA. The PCR-based tech? nique yielded results comparable to those obtained by the conventional pairings. Several restriction pat? terns not previously reported in North American iso? lates were found using the enzyme Alu I. Collections from eight different host species were identified as A. ostoyae, A. sinapina, A. nabsnona, A. gallica, or North American biological species (NABS) XI. Ar? millaria ostoyae, A. sinapina, and A. nabsnona were the most often collected, with the first two occurring predominantly on gymnosperms and the latter most? ly on angiosperms. Armillaria gallica and NABS XI were collected rarely and only from angiosperms. This is only the second report of the occurrence of NABS XI.