Are capacitation or calcium ion influx required for the human sperm acrosome reaction?

Abstract

To determine if the acrosome reaction of human spermatozoa can occur without prior incubation to induce capacitation or in calcium-free medium. Noncapacitated (washed ejaculated) or capacitated (incubated for 3 hours in the presence of albumin) human spermatozoa were treated with either solubilized zonae pellucidae (ZP); a calcium ionophore (A23187); or activators of protein kinases A, G, and C, and the acrosomal status was monitored by the double stain technique. During agonist treatment, the capacitated spermatozoa were in medium either with or without calcium ions (Ca2+). The noncapacitated spermatozoa were always in Ca(2+)-containing medium. Sperm motility was monitored throughout the experiments. Solubilized ZP and kinase activators induced acrosomal exocytosis of capacitated spermatozoa (both in Ca(2+)-containing and Ca(2+)-free medium) and of noncapacitated spermatozoa. The lack of added Ca2+ or capacitation reduced the percentage of spermatozoa that reacted in response to solubilized ZP but not to the kinase activators. Ionophore A23187 stimulated acrosomal loss from noncapacitated spermatozoa to the same extent as capacitated spermatozoa in Ca(2+)-containing medium but had no effect on capacitated spermatozoa in Ca(2+)-free medium. Human spermatozoa do not require incubation under capacitating conditions or the presence of extracellular Ca2+ before they can undergo the acrosome reaction in response to certain agonists. Therefore, Ca2+ influx and/or preincubation to induce capacitation are not absolute requirements for the in vitro agonist-induced acrosome reaction. However, these conditions can optimize the acrosome reaction response to zona proteins. The intracellular mechanisms leading to the acrosome reaction appear to be functional in noncapacitated spermatozoa but membrane changes probably are required before certain extracellular ligands such as zona proteins can exert their maximal effect.