Are C-KIT, MMP-9 and Type II Collagen Positive Undifferentiated Cells Involved in Cartilage Growth? A Description of Unusual Interstitial Type of Cartilage Growth

Abstract

Cartilages growth requires activation of perichondrial cells and chondrocyte multiplication. Activated perichondrial cells produce an outer cartilage matrix and in appositional growth and chondrocytes division produce new chondrogenic cells which participate in interstitial growth of cartilage. This study was performed to identify a third type of cartilage growth in skeletal elements of quail embryos at 6, 7, 8, 9, 10, 13, 16, 17 days of incubation. Paraffin sections of the whole embryos were stained using HE characterized by their small size, having multiple cell processes and staining positive for C-KIT. MMP-9 positive cells were detected in cartilage matrix. The mesenchymal cells further differentiated into chondrocytes which were positive for type II collagen immunostaining and secreted Safranin O positive cartilage matrix. In conclusion, our results suggest that C-KIT positive mesenchymal cells secrete MMP-9 to penetrate growing cartilage, produce type II collagen and proteoglycan and have a role in interstitial growth of embryonic cartilage templates in quail. (Graphical Abstract).