Abstract
In recent year, Aptamer has been one of the key tools in the field of advanced drug delivery systems. Aptamer are oligonucleotides or peptides that bind to a specific target molecule. In this review we summarize the major differences between the antibody and an Aptamer along with the different methodology of the In vitro selection of the Aptamer by using SELEX (Systematic evolution of ligands by exponential enrichment) technique. SELEX is a technique which has a based biosensor and some of the novel drug delivery system. The article referred in this review was referred from the above said source was in the range of 1990-2020 y. Primary contents is searched from science direct, springer nature, scopus indexed journals. The resources are downloaded from google scholar, peer-reviewed published literature from scientific journals and books.
Highlights
Aptamer are single stranded folded oligonucleotides and peptide that binds to molecular protein, which target with the high affinity and specificity because of their specific three-dimensional structure [1, 2]
DNA Aptamer, which is bound to the neurotoxin type B is developed by using Continuous Flow Magnetic Activated Chip Based Separation [CMACS] in which within the single round the Aptamer is selected and the above this used for the development of the Aptamer is designed by the soh’s group and the screening of C-reactive protein (CRP) specific Aptamer is performed by using microfluidic system and magnetic bead conjugated with CRP [18, 19]
This method is the combination of the three-dimensional [3D] cell culture and cell SELEX method, which is used for the development of the appropriate Aptamer against the target molecule
Summary
Aptamer are single stranded folded oligonucleotides and peptide that binds to molecular protein, which target with the high affinity and specificity because of their specific three-dimensional structure [1, 2]. SELEX is the in vitro selection technique which is used to isolate the appropriate Aptamer for the given target. In the binding and separation steps of SELEX the principle used is affinity chromatography in which the library component, which is having the higher affinity towards the target which is being immobilized on the beads is selected
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