Appraising growth, oxidative stress and copper phytoextraction potential of flax (Linum usitatissimum L.) grown in soil differentially spiked with copper

Abstract

Flax (Linum usitatissimum L.) is one of the oldest predominant industrial crops grown for seed, oil and fiber. The present study was executed to evaluate the morpho-physiological traits, biochemical responses, gas exchange parameters and phytoextraction potential of flax raised in differentially copper (Cu) spiked soil viz (0, 200, 400 and 600 mg Cu kg−1 soil) under greenhouse pot experiment. The results revealed that flax plants were able to grow up to 400 mg kg−1 Cu level without showing significant growth inhabitation while, further inference of Cu (600 mg kg−1) in the soil prominently inhibited flax growth and biomass accumulation. Compared to the control, contents of proline and malondialdehyde (MDA) were increased by 160.0% and 754.1% accordingly, at 600 mg Cu kg−1 soil level. The Cu-induced oxidative stress was minimized by the enhanced activities of superoxide dismutase (SOD) by 189.2% and guaiacol peroxidase (POD) by 300.8% in the leaves of flax at 600 mg Cu kg−1 soil level, compared to the untreated control. The plant Cu concentration was determined at 35, 70, 105 and 140 days after sowing (DAS) and results depicted that 16.9 times higher Cu concentration was accumulated in flax roots while little (14.9 times) was transported to the shoots at early stage of growth, i.e. 35 DAS. While at 140 DAS, Cu was highly (21.7 times) transported to the shoots while, only 12.3 times Cu was accumulated in the roots at 600 mg Cu kg−1 soil level, compared to control. Meanwhile, Cu uptake by flax was boosted up to 253 mg kg−1 from the soil and thereby extracted 43%, 39% and 41% of Cu at 200, 400 and 600 mg Cu kg−1 soil level, compared to initial Cu concentration. Therefore, study concluded that flax has a great potential to accumulate high concentration of Cu in its shoots and can be utilized as phytoremediation material when grown in Cu contaminated soils.