Abstract
Energetic Bacillus subtilis was preliminarily isolated from feather meal selection medium experiment, which could be used to produce Keratinase. Through using the ultraviolet ray produced by ultraviolet light and the compound mutation of sodium nitrite solution, a mutated strain was produced which can yield Kerati-nase with a high activity. The activity of Keratinase was 75.9% higher than that before the compound mutation. This was achieved by optimizing the fermentation medium of mutated strain. The optimum fermentation medium had the parameters as feather meal 5.5%, maize silage 0.8%, K+ 0.018 mol/L, Mg2+ 0.065 mol/L, Ca2+ 0.072 mol/L, Fe2+ 0.010 mol/L, and Na+ 0.088 mol/L. Based on the optimized fermentation medium, the highest yielding rate for producing enzyme was 1.117 U/mL, 25.22% higher than that before the optimization. The amino acid in the fermentation medium fluid reached 22.66 mg/mL. This paper presents a simple, and low cost way to produce high quality bio-fermentation feather meal.
Highlights
Poultry feather was often used to feed animals since it was mostly composite of Stearic keratin
When the macromolecule Keratoprotein of feather meal was degraded into small molecular by Bacillus subtilis fermentation which was easier to digeste for animals, new amino acids, bacterial protein, growth factors, immune substance, and beneficial active cell were produced, which provided protein sources for animal growth, and could provide the balanced amino acids content and high nutrition value
The strains of E, F, H, and M are better for degrading feather Keratin, which will be used for the secondary screening
Summary
Poultry feather was often used to feed animals since it was mostly composite of Stearic keratin. The percentage of Stearic keratin in Poultry feather was around 75% 85%. The amino acids in feather Keratin were mostly Hydrophobic amino acids, especially, in which Cystine disulfide bond was difficult to be hydrolyze by Protein hydrolase, so, the activity of feather Keratin was low [1]. If the poultry feather was used to feed animals directly, it will be very difficult for animal to absorb the nutrition. When the macromolecule Keratoprotein of feather meal was degraded into small molecular by Bacillus subtilis fermentation which was easier to digeste for animals, new amino acids, bacterial protein, growth factors, immune substance, and beneficial active cell were produced, which provided protein sources for animal growth, and could provide the balanced amino acids content and high nutrition value
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
