Abstract
The Animal Research: Reporting of In Vivo Experiments (ARRIVE) guidelines were developed to address the lack of reproducibility in biomedical animal studies and improve the communication of research findings. While intended to guide the preparation of peer-reviewed manuscripts, the principles of transparent reporting are also fundamental for in vivo databases. Here, we describe the benefits and challenges of applying the guidelines for the International Mouse Phenotyping Consortium (IMPC), whose goal is to produce and phenotype 20,000 knockout mouse strains in a reproducible manner across ten research centres. In addition to ensuring the transparency and reproducibility of the IMPC, the solutions to the challenges of applying the ARRIVE guidelines in the context of IMPC will provide a resource to help guide similar initiatives in the future.
Highlights
In 2010, the Animal Research: Reporting of In Vivo Experiments (ARRIVE) guidelines were published [1] to address the growing concerns with poor experimental design and lack of transparent reporting of in vivo experiments in the published literature [2,3,4]
We provide an explanation of the analysis, which can be accessed from the International Mouse Phenotyping Consortium (IMPC) ARRIVE landing page
Applying the ARRIVE guidelines for a phenotype resource generated by an international community introduced many challenges, some specific to a data resource and others that are more general
Summary
IMPReSS provides the framework for transparency in the procedure and what data are required to be captured and stores information to facilitate subsequent analysis, making it the backbone of the database and web portal The development of this resource has required extensive collaboration with the institutes, area experts, and database engineers. All strains generated by the IMPC are available to the research community via the established mouse repositories (e.g., Knockout Mouse Repository) These repositories include details about allele structure, genetic background, pathogen exclusion list and any potential issues in husbandry and welfare that may result from carried mutations. This information increases the likelihood of reproducing previous results, which is important for translating mouse research to humans [5]. Lines with interesting disease relationships can be ordered for further research into the pathobiology of the disease and drug target validation
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