Abstract
Chromosome walking technique is an important technique in molecular biology research.Chromosome walking technique by its means is divided into genomic library combined technology and PCR-based technology.As the chromosome walking technique may clone the flanking sequences adjacent to a known sequence,this technique is mainly used in cloning the promoter of known sequence and flanking regulatory element,identifying T-DNA transposon and insertion site of the transposon,discovering upstream and downstream sequence of specific genomic site,gap filling in artificial chromosome sequencing as to obtain the complete genome sequence.As chromosome walking technology continues to improve,it is widely used in genomics and molecular biology.In this review,we demonstrate some key applications of chromosome walking techniques in recent years. Key words: Chromosome walking ; Inverse PCR ; Transposon ; Flanking sequences
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
