Application of Ultrasound-Assisted Extraction Followed by Solid-Phase Extraction Followed by Dispersive Liquid-Liquid Microextraction for the Determination of Chloramphenicol in Chicken Meat

Abstract

In the present study, a simple and efficient preconcentration method was developed using ultrasound-assisted extraction followed by solid-phase extraction followed by dispersive liquid-liquid microextraction for the extraction and determination of trace amount of chloramphenicol in chicken meat. The sample was extracted with acetonitrile and EDTA-McIlvaine buffer under ultrasonication, followed by solid-phase extraction and dispersive liquid-liquid microextraction. In order to obtain high extraction efficiency, the parameters affecting the proposed method were evaluated and optimized. Under the optimized conditions, the calibration curve was linear in the range of 0.3–200 μg kg−1 with good linearity (r 2 > 0.995). Finally, applicability of the proposed method was successfully confirmed by extraction and determination of the chloramphenicol in chicken meat samples. Comparing to the traditional methods, the proposed method exhibits high sensitivity and high preconcentration as well as good precision.