Abstract
The biochemist, concerned with metabolic characteristics of specific cell types or of specific subcellular organelles, is all too frequently limited by the sensitivity of the methods for the assay of a particular chemical or enzyme constituent. This limitation in sensitivity is a function of many variables and would include (1) the inherent sensitivity of the physical technique used for measurements (pH, absorbance, fluorescence, radioactivity, and the like), (2) the concentration or activity of the component being studied, and (3) the size of tissue sample available for study (gram vs. microgram). The latter points may apply particularly to the problems encountered in an attempt to study the enzymatic and chemical constituents of cell types present in the nervous system [1–4].
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