Abstract
A method for assessment of integral biological toxicity of carbon-based nanomaterials (CBN) using a bacterial luminescent biosensor was developed, which accounted for the properties of the objects under study. The proposed approach includes a special procedure for the preparation of the analyzed CBN samples aimed at obtaining highly dispersed suspensions, an extended period of dynamic monitoring of the luminescence of the contacting sensor microorganism, and the special algorithm for quantification of the results of the bioluminescence analysis, in order to exclude the effect of the optical properties of CBN on the results of investigation. This method was used for assessment of the biological toxicity of a broad spectrum of CBN, such as single- and multi-walled nanotubes, nanofibres, and C60- and C70-fullerenes, in order to compare them in this respect to amorphous (nonstructured) carbon.
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