Abstract
ABSTRACT In this paper, the application of the Adsorptive Stripping Voltammetry-Response Surface Methodology (AdSV-RSM) in the simultaneous determination of copper ions in an aquaponics system has been studied. The effects of several parameters studied include variations in Calcein concentration, pH, accumulation potential, and accumulation time. The study design used was Response Surface Methodology (RSM) with 4 factors/variables, 3 levels and 31 treatment combinations. The results of the study, analysis of variance was carried out, and it was decided to accept the second-order model, which means that the independent variable has a significant effect on the response variable (peak current). Based on data analysis with the RSM, the determination of Cu(II) obtained the optimum conditions including peak current 349.196 (Ip); calcein concentration 0.616339 µg/L; pH 6.06208; potential accumulation −0.721104 V and time accumulation of 69.3744 s. The response surface method has been applied successfully for Cu(II) analysis in water, fish, and hydroponic plant aquaponics system, which is an integration between fish farming and hydroponic plant cultivation, with the relative standard deviation value was 1.12%, recovery of 99.23% and the detection limit of 0.75 μg/L.
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