Abstract
Scaffolds are a promising approach for spinal cord injury (SCI) treatment. FGF-2 is involved in tissue repair but is easily degradable and presents collateral effects in systemic administration.In order to address the stability issue and avoid the systemic effects, FGF-2 was encapsulated into core-shell microfibers by coaxial electrospinning and itsin vitro and in vivo potential were studied.Materials & methods:The fibers were characterized by physicochemical and biological parameters.The scaffolds were implanted in a hemisection SCI rat model.Locomotor test was performed weekly for 6 weeks. After this time, histological analyses were performed and expression of nestin and GFAP was quantified by flow cytometry.Results: Electrospinning resulted in uniform microfibers with a core-shell structure, with a sustained liberation of FGF-2 from the fibers. The fibers supported PC12 cells adhesion and proliferation. Implanted scaffolds into SCI promoted locomotor recovery at 28days after injury and reduced GFAP expression. These results indicate the potential of these microfibers in SCI tissue engineering. [Formula: see text].
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