Abstract
The paper shows the possibility of assessing the functional state of microcirculatory-tissue systems of patients with diabetes mellitus by laser Doppler flowmetry (LDF), diffuse reflectance spectroscopy (DRS) and fluorescence spectroscopy (FS) methods. A review of the existing non-invasive optical technologies used to assess the state of microcirculation and oxygen metabolism in tissues of patients with diabetes is conducted. A series of experimental studies involving 76 patients with diabetes and 46 healthy volunteers was carried out. A wavelet analysis of LDF-grams was used to evaluate the adaptive changes of microcirculation during the temperature tests. The obtained data revealed that the proposed methodology in the form of combined use of several diagnostic technologies (LDF, FS and DRS) allows us to detect the presence or absence of trophic disorders and to evaluate adaptation processes during thermal tests.
Highlights
One of the acute problems of modern medicine is the diagnosis of diabetes mellitus (DM)complications
The paper shows the possibility of assessing the functional state of microcirculatorytissue systems of patients with diabetes mellitus by laser Doppler flowmetry (LDF), diffuse reflectance spectroscopy (DRS) and fluorescence spectroscopy (FS) methods
This fact can be explained by the accumulation of advanced glycation end products and formation of glycated proteins, including collagen, which is the largest contributor to the fluorescence spectrum at these wavelengths, in the body of patients with diabetes
Summary
One of the acute problems of modern medicine is the diagnosis of diabetes mellitus (DM)complications. It is known that during diabetes, vascular walls and nerve endings suffer damage due to metabolic disorders, lack of oxygen in the tissues and an excess of toxic products of carbohydrate metabolism. 1.1 Spectrophotometric techniques The diffuse reflectance spectroscopy (DRS) method has been applied in the assessment of tissue saturation. This method allows to noninvasive continuous measure of changes in the concentration of oxygenated haemoglobin (cHbO2) and deoxygenated haemoglobin (cHb). The level of oxygen saturation of tissues (including disorders in diabetes) is judged by the view of the diffuse reflection spectrum based on different mathematical models or empirical relationships derived for communication the reflection coefficient with a concentration of specified chromophores.
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