Abstract
OBJECTIVE: To study and establish quality con-trol model of the Salvianolic Acid B by Near In-frared Spectroscopy (NIRS), and to realize on-line quality control of extracting and purifying proc-esses of industrial scale herbal product manu-facturing. METHOD: NIR chromatography was obtained from on-line NIR detection of extract-ing process and purifying process. HPLC analysis was carried out to determine the con-tents of salvianolic acid B. Partial Least Squares Regression (PLS) was used to establish the model between the information between NIRS and HPLC. RESULTS: For extracting model: the optimum Near Infrared (NIR) wavelength range was 9815- 5430cm-1, R=0.9784, RMSEC=0.258; for puri-fying model: the optimum NIR wavelength range was 9815-5430cm-1, R=0.9776, RMSEC=4.02. The average relative error was <5%. CONCLUSION: NIR technique is applicable for on-line quality control in production of salvianolic acid B.
Highlights
Real-time control of contents has ground to gain in controlling mode of Chinese medicine manufacturing in China, as the very traditional techniques remain the mainstream
The extract and refine devices were shown in the following illustration: 2) On-line collection of Near infrared (NIR) chromatography data 2 parallel chromatographic charts are collected at 2-5min intervals in the process of extracting and refining
Samples for HPLC tests are collected at the same time of collecting NIR chromatography
Summary
Real-time control of contents has ground to gain in controlling mode of Chinese medicine manufacturing in China, as the very traditional techniques remain the mainstream. Off-line tests do not meet the on-line control demands for production process. On-line test is the solution to quality consistency, which is still the bottle-neck in manufacturing of Chinese medicine. Near infrared (NIR) Spectroscopy technology develops rapidly in recent years as a fast analytical technology. It shows the substances and structures of the tested samples indirectly. The procedure of acquiring information by NIR chromatography is easy and low-cost. It does not require complicated pre-treatment of the samples but still meets the requirement of on-line testing
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