Application of fetal limbal stem cell in the treatment of pseudopterygium

Abstract

Objective To study the clinical effect of fetal limbal stem cell in the treatment of pseudopterygium.Methods 16 eyes of 16 patients had been peformed the operation of pseudopterygium excision combined with fetal limbal stem cell transplantation.Fresh human fetal limbal stem cell with donors' gestational age of 28 weeks or more were used intraoperatively.The medicine preventing immune rejection were used after operation.Corneo-conjunctival healing,immune rejection,palindromia of pterygium and intraocular pressure were observed in the follow-up.Results The patients were followed up for (9.4 ± 2.4)months.The average time for the corneal epithelial healing was (3.8 ± 1.3) d.In the follow-up,13 cases (81.3 ％) of corneal epithelial continued complete,1 case (6.3 ％) recurred pseudoperygium.The number of epithelial rejection were 7 cases,3 cases,0 case and 0 case at 1 m,3 ms,6 ms and at the last follow-up time after operation,but the rejection was controlled through medication,the average time of medicine treatment was (3.9 ± 1.3) d.At 1,3,and 6 months after surgery and the last follow-up,the mean rating value of graft rejection were 0.6 ± 0.9,1.0 ± 1.1,0.3 ± 0.8,0.3 ± 0.8.Six months after operation,there were 3 cases of graft dissolution.Two cases were significantly improved by anti-rejection medication,another one had continued dissolution and the graft failed to survive.Conclusion Preliminary results are satisfactory for the treatment of pseudopterygium caused by physic and chemical damage through transplanting fetal limbal stem cell,it provides a new idea for the clinical treatment of those diseases. Key words: Fetal; Stem cell, limbal; Tissue transplantation; Pseudopterygium